Figure 3.

TLR2/4 are crucial for repeated social defeat stress-induced PGE₂ synthesis in the brain. (a) Schedule of experiments in this study. After social isolation for 1 week, a male wild-type or TLR-DKO mouse was subjected to SDS through an encounter with an ICR aggressor mouse for 10 min daily for 10 consecutive days (Day 10). The measurement of PGE₂ and IL-1β was performed immediately after the last SDS exposure on Day 10. (b,c) The contents of PGE₂ (b) and IL-1β (c) in whole brains of wild-type (WT) and TLR2/4 double knockout (TLR-DKO) mice treated with or without repeated SDS (R-SDS). The contents of PGE₂ and IL-1β were normalized to the protein concentrations. *P < 0.05 for Bonferroni’s multiple comparisons test. Data are shown as means ± SEM.