Figure 5.

JZL184, an inhibitor for MAGL, suppresses social defeat stress-induced social avoidance. (a) Schedule of experiments. After social isolation for 1 week, a male wild-type mouse was subjected to SDS through an encounter with an ICR aggressor mouse for 10 min daily for 10 consecutive days (Day 1 through Day 10). JZL184 or vehicle was administered at 6 h before each SDS exposure. The social interaction test and the elevated anxiety test were performed after repeated SDS to analyze repeated SDS-induced social avoidance and elevated anxiety, respectively. (b,c) The effect of JZL184 on repeated SDS-induced social avoidance. The durations in the interaction (b) or avoidance (c) zone without or with an ICR mouse during the social interaction test were measured for wild-type mice without SDS (naïve) or with repeated SDS (R-SDS) which received i.p. injection of either vehicle or JZL184. (d) The effect of JZL184 on repeated SDS-induced elevated anxiety. The proportions of the time spent in the closed arms, open arms and center zone in the elevated plus maze test were measured for the same mice analyzed for the social interaction test. ^#P < 0.1, *P < 0.05, **P < 0.01 for Bonferroni’s multiple comparison test. Data are shown as means ± SEM.