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. 2019 Nov 26;9:17558. doi: 10.1038/s41598-019-54175-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Anti-IL-6Rα antibody protection against postoperative adhesion formation. Wild-type mice were treated with various doses of anti-IL-6Rα antibody or isotype-matched antibody and subjected to cecum cauterization. (a) Adhesion scores were determined on day 7 following the procedure. Representative photos are shown. (b,c) Cecum lesions were sampled at the indicated time points for indicated protein staining (b) and for qRT-PCR analysis of indicated proinflammatory and pro-fibrotic mRNA transcripts (c). Each experimental group contained 3–5 mice, with two independent experiments performed. Representative photos are shown. Data at 0 day or hour postoperation indicated those in untreated control mice. Data are shown as mean ± SEM. *p < 0.05 (Student’s paired t-test). Asma, gene for αSMA.