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. 2019 Nov 26;8(12):70. doi: 10.1038/s41389-019-0179-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Collection of conditional medium form HeLa229P or HeLa229/TR cells with or without 5 μM erlotinib treatment for 48 h to culture HeLa229P or HeLa229/TR cells, then flow cytometry assay was applied to detect the percentage of CD133⁺ cells. b RT-qPCR (upper) and ELISA (lower) analysis of indicated cytokines in HeLa229P and HeLa229/TR cells after treated with 0 μM or 5 μM erlotinib for 48 h. c Sphere-formation assay and colony-forming assay were performed in HeLa229P and HeLa229/TR cells treated with DMSO or IL-6 neutralizing antibody (10 μg/ml) for 48 h (scale bar = 100 µm). Data represent mean ± SD from three independent experiments (n = 3). Differences between linked groups were evaluated by two-tailed Student's t test. **P < 0.01; ***P < 0.001; ns not significant, P parental, TR paclitaxel-resistant, CM conditional medium, Erl erlotinib, αIL-6 IL-6 neutralizing antibody.