Fig. 6. Nfκb2^−/− enteroids showed a blunted response to co-culture with LPS activated BMDCs.

Enteroids were generated from the proximal SI of C57BL/6J, Nfκb1^−/− and Nfκb2^−/− mice and were either left untreated, treated with 1 µg/ml LPS, 5 × 10⁴ C57BL/6J-derived BMDC or 5 × 10⁴ C57BL/6J-derived BMDC plus 1 µg/ml LPS and subjected to light microscopy at 48 h (a). Circularity assessment was conducted at 0, 24 and 48 h time points for each stimulus in C57BL/6J (b), Nfκb1^−/− (c) and Nfκb2^−/− (d) enteroids. N = 3, n = 6, *p < 0.05, **p < 0.01 compared with untreated of the same enteroid genotype at the same time point. Scale bars = 100 µm.