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Serum HSP70 immunoreactivity. Pooled sera (diluted 1:300) from SHR-A3 and SHR-A3(IgH-B2) rats were tested for immunoreactivity to HSP70 by dot blot. Serial dilutions of rat recombinant HSP70 (starting concentration 0.68 μg/ml) were spotted on to nitrocellulose membranes, and serum immunoreactivity was detected with secondary antibodies to IgG (left) and IgM (right).
