Fig. 5.

Liver-specific antisense oligonucleotides (ASOs) in HF animals result in a modest fall in blood pressure. Dahl S animals receiving high-fat (HF) diet and the liver-specific ASO displayed a modest fall in mean arterial pressure (MAP) that did not approach the large fall in blood pressure observed with the whole body ASO (A). Heart rate (HR) did not change with any of the treatments (B). Pulse pressure (PP) was significantly decreased with the whole body ASO but not the liver-specific ASO (C). Dotted vertical lines represent dates of ASO injection and dotted horizontal lines represent baseline. Baseline values are listed in Supplemental Table S1 (https://figshare.com/s/ccd53be88eaefd81f466). Points and error bars represent means ± SE. *P < 0.05 of the HF whole body group compared with all other groups; +P < 0.05 of the HF liver-specific ASO group compared with all other groups. HF whole body ASO and control ASO data were pooled with matching HF data from Fig. 3. Statistics were measured by 2-way ANOVA and Tukey’s multiple-comparison test. n values represent biological replicates and are listed in the figures.