Fig. 3.
Peroxisome proliferator-activated receptor-α (PPARα) hepatocyte-specific knockout (PparaHepKO) mice have enhanced high-fat diet (HFD)-induced hepatic steatosis. A: Oil red O staining in livers from PPARα wild-type (Pparafl/fl) and PparaHepKO mice on normal fat diet (NFD) and HFD. B: biochemical measurement of hepatic triglyceride levels. C: hepatic fat mass as measured by noninvasive EchoMRI at the end of the study. D: hepatic lean mass as measured by noninvasive EchoMRI at the end of the study. Measurement of hepatic metabolic gene expression in NFD and HFD in Pparafl/fl and PparaHepKO mice for G0s2 (E), Ctp1a (F), and peroxisomal gene expression for Abcd3, Acox1, Crot, and Pex7 (G). Values are means ± SE; *P < 0.05 vs. NFD Pparafl/fl; #P < 0.05 vs. HFD Pparafl/fl; ^P < 0.05 vs. NFD PparaHepKO. A: n = 9 and 8 Pparafl/fl and PparaHEPKO NFD and HFD mice. B: n = 6 mice. C and D: n = 16 and 13 Pparafl/fl NFD and HFD mice and n = 6 and 8 PparaHepko NFD and HFD mice. E–G, n = 16 and 8 Pparafl/fl NFD and HFD mice and n = 6 and 8 PparaHepKO NFD and HFD mice. All circles, NFD; all squares HFD.