Fig. 5.
Mice with a loss of hepatic peroxisome proliferator-activated receptor-α (PPARα) on a high-fat diet (HFD) have higher triglycerides and apolipoprotein B100 levels. A: plasma triglycerides as measured by NMR spectroscopy. Pparafl/fl, PPAR wild-type; PparaHepKO, PPARα hepatocyte-specific knockout mice. B: plasma triglyceride distribution as measured by NMR spectroscopy. Plasma ApoB100 as measured by NMR spectroscopy. C: plasma ApoB100 as measured by NMR spectroscopy. mRNA levels in livers from Pparafl/fl and PparaHepKO mice on normal fat diet (NFD) and HFD: Apob and Mttp (D), Npc2 (E), and Ldlr (F). Values are means ± SE; *P < 0.05 vs. NFD Pparafl/fl; #P < 0.05 vs. HFD Pparafl/fl; ^P < 0.05 vs. NFD PparaHepKO; n = 6 and 12, Pparafl/fl NFD, HFD; n = 6 and 8, PparaHepKO NFD, HFD; A and C, n = 8 and 12; B, n = 16 and 8 Pparafl/fl NFD, HFD; D, E, and F, n = 6 and 8, PparaHepKO NFD, HFD. n, number of mice. All circles, NFD; all squares, HFD.