Fig. 6.

Transcriptional regulation of de novo lipogenesis (DNL) during relapse. RNA-sequencing data from relapsing rats were mined to determine differential expression of genes that encode transcription factors that mediate DNL. A: association between hepatic carbohydrate-response element-binding protein (Chrebp) expression and net hepatic DN lipid retention (n = 15), as determined by Pearson’s product-moment correlation. B: mRNA expression of Chrebp. C: plasma glucose adapted from the initial report of the dual-tracer study, originally published in Am J Physiol Regul Integr Comp Physiol 301: R656–R667, 2011 (43). D: mRNA expression of sterol regulatory element-binding protein 1 (Srebp1). E: mRNA expression of upstream stimulatory factor 1 (Usf1). F: mRNA expression of liver X receptor β (Lxrβ). Data were analyzed by ANOVA, with planned comparisons (n = 5 per group for gene expression data, n = 7–12 for plasma glucose data; *P < 0.05; **P < 0.01). EX, exercise; FPKM, Fragments Per Kilobase of transcript per Million mapped reads; GM, gap-matched; REL, relapse; SED, sedentary; WLM, weight loss maintenance.