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. 2019 Sep 10;317(5):E839–E851. doi: 10.1152/ajpendo.00151.2019

Table 5.

Relative abundances of phosphorylated signaling proteins in visceral organs and brain of fasted or fed piglets born at preterm or term gestation

Preterm
 Term
 P Value
Tissue (Protein) Fasted Fed Fasted Fed GAB Feed GAB × Feed
Lung
    4EBP1 Thr70 0.08 ± 0.01 0.56 ± 0.04 0.07 ± 0.01 0.60 ± 0.04 NS <0.001 NS
    S6K1 Thr389 0.08 ± 0.01 0.69 ± 0.05 0.08 ± 0.01 0.72 ± 0.04 NS <0.001 NS
    PKB Ser473 0.14 ± 0.02 0.63 ± 0.04 0.13 ± 0.01 0.62 ± 0.04 NS <0.001 NS
Jejunum
    4EBP1 Thr70 0.14 ± 0.03 0.72 ± 0.03 0.13 ± 0.04 0.77 ± 0.04 NS <0.001 NS
    S6K1 Thr389 0.13 ± 0.04 0.59 ± 0.04 0.11 ± 0.04 0.55 ± 0.04 NS <0.001 NS
    PKB Ser473 0.14 ± 0.04 0.59 ± 0.04 0.13 ± 0.04 0.62 ± 0.04 NS <0.001 NS
Liver
    4EBP1 Thr70 0.10 ± 0.01c 0.67 ± 0.04a 0.09 ± 0.01c 0.48 ± 0.03b 0.03 <0.001 <0.001
    S6K1 Thr389 0.10 ± 0.01c 0.79 ± 0.03a 0.09 ± 0.01c 0.57 ± 0.03b 0.03 <0.001 <0.001
    PKB Ser473 0.13 ± 0.01c 0.77 ± 0.04a 0.10 ± 0.01d 0.50 ± 0.03b 0.01 <0.001 <0.001
Pancreas
    4EBP1 Thr70 0.14 ± 0.02c 0.49 ± 0.02b 0.15 ± 0.02c 0.81 ± 0.02a <0.001 <0.001 <0.001
    S6K1 Thr389 0.08 ± 0.03c 0.34 ± 0.03b 0.09 ± 0.03c 0.65 ± 0.03a 0.005 <0.001 <0.001
    PKB Ser473 0.13 ± 0.04c 0.44 ± 0.04b 0.12 ± 0.04c 0.69 ± 0.04a 0.05 <0.001 <0.001
Kidney
    4EBP1 Thr70 0.12 ± 0.02c 0.45 ± 0.02b 0.13 ± 0.03c 0.72 ± 0.03a 0.003 <0.001 <0.001
    S6K1 Thr389 0.09 ± 0.03c 0.49 ± 0.03b 0.12 ± 0.03c 0.77 ± 0.03a 0.005 <0.001 <0.001
    PKB Ser473 0.09 ± 0.05c 0.35 ± 0.05b 0.08 ± 0.06c 0.59 ± 0.06a NS <0.001 <0.001
Brain
    4EBP1 Thr70 0.11 ± 0.03 0.48 ± 0.03 0.10 ± 0.03 0.51 ± 0.03 NS <0.001 NS
    S6K1 Thr389 0.13 ± 0.03 0.58 ± 0.03 0.15 ± 0.04 0.63 ± 0.04 NS <0.001 NS
    PKB Ser473 0.23 ± 0.05 0.75 ± 0.05 0.18 ± 0.05 0.70 ± 0.05 NS <0.001 NS

Values are least square means ± SE; n = 21–25/group. Values are expressed as arbitrary units. 4EBP1, eukaryotic initiation factor 4E binding protein 1; feed, feeding status; GAB, gestational age at birth; NS, not statistically significant, P > 0.05; PKB, protein kinase B; S6K1, S6 kinase 1. Two-way ANOVA followed by Tukey’s post hoc test.

a,

b,

c,

d

Means without a common superscripted letter differ, P < 0.05.