Fig. 1.

RT-PCR in porcine choroid plexus-Riems (PCP-R) cells. A: mRNA for the various cytokine receptors. All were present except for the second isoform of the IL-1 receptor (Il1r2) and the IL-10 receptor (Il10ra). B: mRNA for porcine Toll-like receptors (TLR) 1–10 is present in the PCP-R cell line. C: RT-PCR results for various enzymes capable of arachidonic acid (AA) metabolism. Cytochrome P (CYP)450 epoxygenases, Cyp2c42 and Cyp2e1, are both absent. mRNA for lipoxygenase (Alox)15 and Alox15b are present whereas Alox5 and Alox12 are absent. Cyclooxygenase (Cox)1 and 2 are both present. Gapdh was utilized as positive controls for each gel. Band product sizes can be found in Table 2. Ladder, 100bp; T, template. Lanes denoted (+) or (−) “T” signify addition or no addition of template control. D: diagram of AA metabolism via the three different pathways, pathway inhibitors, and subsequent metabolites.