Fig. 2.

Pretreatment of porcine choroid plexus-Riems (PCP-R) cells with proinflammatory cytokine IL-1β for 24 h prior to transient receptor potential vanilloid 4 (TRPV4) agonist addition. Effect on transepithelial ion flux and conductance was measured. IL-1β (10 ng/mL in PBS) was added to the PCP-R media on both basolateral and apical sides of the membrane at time (t) = −24 h (not shown). TRPV4 agonist GSK1016790A was added to basolateral side of the membrane only at t = 0 min. Incubation for 24 h with IL-1β is signified by white circles. GSK1016790A control is signified by black circles. Circles signify mean values and bars signify ±SE for number of experiments indicated. SCC, short-circuit current. *P < 0.05 against control.