Fig. 12.

Dose effect of arachidonic acid (AA) on the change in transient receptor potential vanilloid 4 (TRPV4)-mediated transepithelial ion flux in response to TRPV4 agonist GSK1016790A. Porcine choroid plexus-Riems (PCP-R) cells were pretreated with arachidonic acid (AA) solubilized in 100% ethanol at various concentrations 10 min prior to TRPV4 agonist addition. AA was added to the PCP-R media on both basolateral and apical sides of the membrane. The change in transepithelial ion flux was measured 5 min after TRPV4 agonist addition (ΔSCC). Error bars signify the SE for number of experiments indicated. ΔSCC, change in short-circuit current. **, ***, and ****Significant against 0 µM AA control with P values given.