Table 2.
Sus scrofa forward and reverse primer sequences used for RT-PCR with corresponding product sizes (bp)
| Primer Sequence 5′–3′ |
||||
|---|---|---|---|---|
| Sus Scrofa Gene | Protein | Forward Primer | Reverse Primer | Product Size, bp |
| Il1r1 | IL1R1 | TTACACAGAGACTACCGGTTGC | CAGTTTTATCACCCGGGAGACA | 263 |
| Il1r2 | IL1R2 | AAATGATTCCACGACCATCCCA | TCCCAAAACCAGGATGATGAGG | 874 |
| Tnfrsf1a | TNFRSF1A | GAAACAGGACACCATCTGCAAC | AAGCTAAGCCAACGAAGAGGAA | 218 |
| Tgfbr1 | TGFBR1 | GAGACAGGCCATTTGTATGTGC | TAAATCTCTGCCTCACGGAACC | 520 |
| Tgfbr2 | TGFBR2 | GGAAGCTGATGGAGTTCAGTGA | TCTCTGTCTTCCAGGAGGCATA | 252 |
| Tgfbr3 | TGFBR3 | ATATTCACCACAAGCCTGTCGT | ATACTCCTTTCGGGCCCAATTT | 224 |
| Il6r | IL6R | AAGATTCTGCGTCGGTACCATT | TGTTGCTGACATCATAAGGGCT | 312 |
| Il10ra | IL10RA | GGATGAAGTGACTCTGACGGTT | GCTTCACCCTGACACACAATTC | 257 |
| Il4r | IL4R | AGAGCTACCTGTACTCGGAACT | CCCTTTGTCTTCCTCCTCTTCC | 695 |
| Tlr1 | TLR1 | TGTCCCACAACAAGTTGGAGAA | TTGTGGGAAACTGAACACCTCA | 650 |
| Tlr2 | TLR2 | TCCCAAATCTGCGAATCCTGAA | ATGCAACCTCCGGACTGTTAAT | 512 |
| Tlr3 | TLR3 | CGATGACCTCCCGGCAAATATA | GAGATTTTCCAGTTGGAGCTGC | 382 |
| Tlr4 | TLR4 | TTCTCTCCTGCCTGAGATCTGA | ACTCCAGGTAGGTATTCCTGCT | 555 |
| Tlr5 | TLR5 | TCCTGTGGTCTCTCTGATGCTA | GGGTTCATACACTTCCCCCAAT | 284 |
| Tlr6 | TLR6 | AGACAATCTTGTGCCATCCCAT | GGCCCTTGAGTGAGTTCCAATA | 409 |
| Tlr7 | TLR7 | GACACTAAAGACCCAGCAGTGA | CTGAAGGGGCTTCTCAAGGAAT | 297 |
| Tlr8 | TLR8 | CTGAGGCAGAACAGGATTTCCT | TTCATCACCCAGTCTGTGACAG | 542 |
| Tlr9 | TLR9 | GCCTACGAACTCTCAACCTCAA | GGAAGTTCTCACTCAGGTCCAG | 696 |
| Tlr10 | TLR10 | TCAGGTGCTTGCCCAGAAATAT | TCTTGCCAGGATCAGAGTTTCC | 717 |
| Cyp2c42 | CYP2C42 | TCCTGTCTGCTTCTCCTTTCAC | GGGAGCACAGTCCAGGATAAAA | 489 |
| Cyp2e1 | CYP2E1 | TCGAGATTTCACTGACACCCTG | GTTAAAGTGCTGCAAGATGGCA | 592 |
| Alox5 | 5-LOX | TGACAGTGGATGAAGAACTGGG | TGTTTTTGCCGTGTTTCCAGTT | 259 |
| Alox12b | 12R-LOX | ACACCATCCAGATCAACAGCAT | CCAGAGGACCAATAGGACGATG | 602 |
| Alox15 | 12/15-LOX | CAGGAGGATGAACTCTTTGGCT | TCGAATATACCTCCGTCCGAGA | 578 |
| Alox15b | 15-LOX2 | GCGAAATGCTGAGTTCTCCATC | CAGGGTGGAATAGTTCAGCTGT | 283 |
| Cox1 | COX1 | TCACCCGCAATACTATGAGCTC | TGTGTGATAGGGAGGAGGACAT | 510 |
| Cox2 | COX2 | CCCTTTCCAACTAGGCTTCCAA | TAGTCGTCCTGGGATAGCATCT | 529 |
| Gapdh | GAPDH | TTTTAACTCTGGCAAAGTGGAC | CATTGTCGTACCAGGAAATGAG | 884 |
Three different primer sets were generated and tested for each gene. Primers included in this table were utilized for Fig. 1, A–C. GAPDH was used as a positive control.