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The absence of carbonic anhydrase III (CAIII) in normal mouse heart. The SDS-gels and Western blots show that mAb CP3 recognizes CAIII in mouse soleus (slow twitch-type) muscle, liver, and fat. The fast twitch-type extensor digitorum longus (EDL) muscle and cardiac muscle show no detectable expression of CAIII. Since mAb CP3 was raised against chicken gizzard smooth muscle calponin 1 immunization, it also recognizes smooth muscle calponin 1 in mouse bladder. MHC, α-myosin heavy chain.
