Fig. 6.

Neonatal Fc receptor (FcRn) knockout (KO) podocytes are hypermotile compared with wild type (WT) and motility can be restored towards normal after treatment with proinflammatory stimuli. A: images of WT and FcRn KO podocytes immediately (time (t) = 0) and 8 h after being scratched, treated with regular media (RM), IFNɣ, IFNɣ plus 4-hydroxy-3-nitrophenylacetyl hapten-ovalbumin (NP-ova), IL-6, NP-ova, or IL-6 plus NP-ova at 0 and 8 h (n ≥ 4 experiments). Scale bar: 300 µm. B: quantitative analysis of the percentage area of the gap closed 8 h after scratching. Data were analyzed by using ImageJ. Data are presented as mean ± SE. In B, ns, not statistically significant, *P < 0.05, **P < 0.01, and ****P < 0.0001.