Fig. 7.

Neonatal Fc receptor (FcRn) knockout (KO) podocytes have decreased stress fibers at baseline compared with wild type (WT) and treatment with interleukin-6 (IL-6) restores stress fiber formation. A and B: actin in untreated WT and FcRn KO podocytes was stained with 635 phalloidin (green). Nuclei were stained with Hoechst (blue). C and D: images were obtained after treatment with IL-6 for 48 h (n = 3 experiments for all conditions). Scale bar: 20 µm. Quantitation of stress fiber length shows that at baseline, FcRn KO podocytes have stress fibers that are significantly shorter than WT. After treatment with IL-6, there are almost no stress fibers in the WT whereas KO podocyte stress fiber length is restored to that of WT. Data are presented as means ± SE from 3 independent experiments. In B, ns, not statistically significant, and ****P < 0.0001.