Skip to main content
. 2019 Aug 21;317(5):L653–L666. doi: 10.1152/ajplung.00503.2018

Fig. 2.

Fig. 2.

Sftpc-rtTA 114/tet-O-Cre/Ai9 line. A: doxycycline (Dox) E10–18 analyzed at PN 4 wk (n = 7). Extensive TdTomato (tdT) expression, more in TII cells (arrows, Sftpc+) than TI cells (arrowheads). Higher magnification views show TII and TI cells. B: Dox E12–15 analyzed at PN 4 wk (n = 3) results in a few TII cells (arrows, Sftpc+) and rare TI cell-like TdTomato+ extensions (arrowheads). Some, not all, Sftpc+ cells are TdTomato+; arrows denote tdt+/Sftpc+ cells; asterisks indicate tdt−/Sftpc cells. The image was selected to show both TI and TII cells in the same field. C–E: Dox E15–18 analyzed at E18 (n = 3). Most TdTomato+ cells are cuboidal and Sftpc+. A few TdTomato+ cells have Pdpn+ (arrowheads) thin cytoplasmic extensions). F: Dox E15–18 analyzed at PN day 7 (d7; n = 3). Most are TdTomato+ cuboidal cells (arrows), with patchy expression of TdTomato in cells with a TI-like morphology (arrowheads) (see Fig. 1G for comparison with RT22). G: Dox E15–18 analyzed at PN 4 wk (n = 8). Most TdTomato+ TII cells are Sftpc+ (arrows); there is patchy TdTomato expression in Pdpn+ TI cells (arrowheads); merged TdTomato+/Pdpn+ areas are orange (arrowheads). E, embryonic day; PN, postnatal.