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. 2019 Aug 21;317(5):L653–L666. doi: 10.1152/ajplung.00503.2018

Fig. 4.

Fig. 4.

Scattergrams and cytocentrifuged preparations of FACS E18 cells in 114 and R22 lineages. In both 114 and R22 lineages, doxycycline (Dox) was administered E15–18, and cells were harvested at E18. A and B: R22E18 cells. A: scattergram showing FACS of R22 E18 cells. There were insufficient numbers of TdTomato+/Pdpn− cells to isolate by these methods. B: cytocentrifuged preparations of each group of R22 E18 cells showing immunofluorescence for TdTomato, Pdpn, and AQP5. C and D: 114 E18 cells. C: scattergram showing FACS of 114 E18 cells. D: cytocentrifuged preparations of each group of 114 E18 isolated cells showing immunofluorescence of the different FACS populations for TdTomato (tdT), Pdpn, and SP-C. The TdTomato+/Pdpn− population is SP-C+. E, embryonic day.