Fig. 5.

Schematic of alveolar epithelial development and potential regulatory factors derived from RNAseq analysis of pre-TI and pre-TII cells. A: schematic of alveolar epithelial development; a: pathway defined in the R22/tet-O-Cre/Ai9 line. At E12–15 and E15–18, a progenitor population gives rise almost completely to TI cells; a very small number of TII cells (~0.1%) is produced by this pathway; b: pathway defined in the 114/tet-O-Cre/Ai9 line. At E12–15 and E15–18, a progenitor population gives rise mostly to TII cells, with a subpopulation of TI cells. B: following RNAseq analysis of pre-TI and pre-TII cells, we analyzed the data for potential regulatory factors important in TI cell development. Bar graphs showing the top 25 (by log₂DE) transcription and GFs common to both populations of pre-TI cells, when each is compared with the pre-TII cell population (E18 114 A9⁺/Pdpn⁻). For each factor, the log₁₀FDR is shown on the left bar graphs and the log₂DE on the right. E, embryonic day.