Fig. 3.

Postseptic pulmonary endothelial cells downregulate sulfatase-1 (Sulf-1) mRNA. A: we collected pulmonary endothelial cells and extracted total RNA at day 2, a time point immediately before glycocalyx reconstitution at day 3. B: pulmonary endothelial cells in mice 48 h after cecal ligation and puncture (CLP) had decreased Sulf-1 expression detected with RNA microarray. C: we validated the downregulation of Sulf-1 in pulmonary endothelial cells by quantitative RT-PCR using separate biological replicates. D: we collected pulmonary endothelial cells and extracted total RNA at 12 h after heparinase III (Hep III) injection, a time point immediately before glycocalyx reconstitution after enzymatic, nonseptic degradation. IV, intravenous. E: mRNA expression level of Sulf-1 in pulmonary endothelial cells were similar 12 h after enzymatic degradation by Hep III or heat-inactivated (HI) Hep III. *P < 0.05 by Student’s t test; n = 3–4 each group.