Fig. 6.

Knocking down sulfatase-1 (Sulf-1) in pulmonary microvascular endothelial cells decreases ICAM-1 expression. A: we transfected cultured endothelial cells with siRNA targeted to Sulf-1 using Lipofectamine RNAiMAX for 24 h, which resulted in greater than 83% knockdown of Sulf-1. B: knockdown of Sulf-1 resulted in downregulation of ICAM-1 expression in endothelial cells. Control cells were treated with lipofectamine without siRNA. *P < 0.05, Student’s t test; n = 3, each data point represents average of 2–3 wells per treatment.