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. 2019 Aug 14;317(5):L525–L536. doi: 10.1152/ajplung.00067.2019

Fig. 4.

Fig. 4.

Moderate hyperoxia suppresses autophagy but not endoplasmic reticulum (ER) stress in primary human fetal lung fibroblasts. A: monodansylcadaverine (MDC) staining of primary fetal lung fibroblasts exposed to normoxia, hyperoxia for 7 days, or to etoposide for 48 h. Tamoxifen was used as a positive control for induction of autophagy (×400 magnification). Graph shows fluorescence measurements of autophagic level (MDC fluorescence). B: representative Western blot using antibodies against autophagy markers LC3B and beclin-1. Graphs represent relative expression of LC3B-II or beclin-1 to GAPDH expression. C: representative Western blot using antibodies against ER stress markers IRE1α, Bip, p-PERK, PERK, and GAPDH. Graphs represent relative expression of IRE1α, Bip, p-PERK, or PERK to GAPDH expression. Values are means ± SE from n = 5–7 samples. *P < 0.05 compared with normoxia group (one-way ANOVA).