Fig. 6.
Effect of moderate hyperoxia on extracellular matrix (ECM) deposition. A modified Li-Cor In-Cell Western technique (semiquantitative immunofluorescence) was used to show deposition of collagen I, III, IV, and fibronectin by fibroblasts exposed to normoxia (21% O2) or hyperoxia (40% O2) for 7 days or treated with etoposide. Hyperoxia increased collagen IV while etoposide increased other ECM proteins as well. Values are means ± SE from n = 7 or 8 samples. *P < 0.05 compared with normoxia group (one-way ANOVA).