Fig. 3.

Effects of hypoxia and cartilage oligomeric matrix protein (COMP) on the expression of the different forms of SOD present in bovine pulmonary artery (BPA). The level of individual forms of SOD measured by Western blotting in BPA cultured under 21% (CTL) or 3% O₂ for 48 h (H48) in the absence and presence of COMP (C, 0.5 μM) is shown. A–C: representative Western blot bands and quantitative analysis of Cu,Zn-SOD1 (A) (n = 8), mitochondrial SOD2 (B) (n = 6), and extracellular SOD3 (C) (n = 6). *P < 0.05, two-way ANOVA with Sidak correction. D: representative Western blot bands and quantitative analysis showing the effect of knockdown of COMP with siRNA decreasing protein levels of SOD2 (n = 9) and SOD3 (n = 7). *P < 0.05, COMP-siRNA vs. scramble siRNA, paired t test. (H48, 3% O₂ for 48 h; H48 + C, 3% O₂ for 48 h and treated with 0.5 μM COMP at the same time; COMPi, siRNA-COMP.) B and C were edited between the 2nd and 3rd channels that are shown separated by a line to remove two additional channel bands from the original imaging capture of the same membrane.