Fig. 1.

Differential Ca²⁺/calmodulin-dependent protein kinase IIγ (CaMKIIγ) expression in differentiated vascular smooth muscle (VSM_Diff) and VSM synthetic phenotype (VSM_Syn). Rat aorta medial layer VSM cells (VSMCs) were enzymatically dispersed and cultured for 3–6 passages. A and B: CaMKIIγ and GAPDH were analyzed by SDS-PAGE and immunoblotting (IB) comparing intact aorta medial layer VSM (VSM_Diff) and primarily cultured aortic VSM cells (VSM_Syn). C–E: quantitative PCR was used to measure Camk2g, Tagln, myosin heavy chain (Myh11), and Gapdh mRNA. All values were normalized over GAPDH expression. Values are shown as means ± SE, n = 3–7, and analyzed by unpaired t test. **P < 0.01 and ***P < 0.001.