Fig. 4.

Camk2g promoter is methylated in vascular smooth muscle synthetic phenotype (VSM_Syn) cells in vivo. A: rat carotid arteries were intraluminally injured, and 14 days later, protein levels of smooth muscle myosin heavy chain (MYH11), Ca²⁺/calmodulin-dependent protein kinase IIγ (CaMKIIγ), thymine DNA glycosylase (TDG), and β-actin were measured by SDS-PAGE and Western immunoblotting (IB) using specific antibodies in uninjured contralateral (UN) and balloon-injured (IN) carotid arteries. B: representative sections were examined by hematoxylin-eosin staining. C: genomic DNA was extracted from uninjured and balloon-injured left CAs 14 days after injury. Enrichment of methylated Camk2g and Myh11 promoters was determined using methylated DNA immunoprecipitation (MeDIP) followed by quantitative PCR. Values are shown as means ± SE, n = 5, and analyzed by paired t test. *P < 0.05.