Fig. 7.
Trehalose treatment decreases calcium sensitization in mesenteric resistance arteries (MRAs) from spontaneously hypertensive rats (SHRs) but impairs endothelium-independent relaxation in MRAs from Wistar rats. A: concentration-response curves to Y-27632 in MRAs from Wistar and SHRs treated with vehicle or trehalose. PE, phenylephrine. B and C: protein expression analysis for Rho kinase (ROCK) II (B) and phospho-myosin phosphatase target subunit 1 (MYPT1; C) in MRAs. Left, densitometric analysis; right, representative images of immunoblots. D: concentration-response curves to SNP in MRAs from Wistar and SHRs treated with vehicle or trehalose, For A–D, n = 5–8. Two-way ANOVA: *P < 0.05 vs. Wistar vehicle, #P < 0.05 vs. SHR vehicle. One-way ANOVA: *P < 0.05 vs. Wistar vehicle, #P < 0.05 vs. SHR vehicle. LogEC50: *P < 0.05 vs. Wistar vehicle.