Fig. 7.

Ischemia-reperfusion injury (IRI) with delayed contralateral nephrectomy (Nx) (DN-IRI model) in male BALB/c mice. A: schematic of the DN-IRI study design. Eleven-week-old male BALB/c mice underwent 30 min of unilateral renal ischemia followed by a contralateral Nx 8 days later (n = 5) or Nx only (n = 5). B: glomerular filtration rate (GFR) calculated from transdermal measurement of FITC-sinistrin clearance at baseline, day 9 (1 day after Nx), and weeks 2, 3, 4, 6, 8, and 12. C: quantification of renal fibrosis [picrosirius red (PSR) staining] in the outer medulla at weeks 4 and 12. D: representative images of the outer medulla of PSR-stained kidney sections. E−G: fold changes of mRNA expression of the following fibrosis markers: α-smooth muscle actin (α-SMA; E), lysyl oxidase-like 2 (LoxL2; F), collagen type I-α₁ (Col1-α1; G), and collagen type III-α₁ (Col3-α1; H) relative to GAPDH at weeks 4 and 12. Bars in B represent means ± SE. Data points in C and E–H represent individual animals, with means ± SE of the groups indicated. Two-way ANOVA (B) or one-way ANOVA (C, E–H) was used to determine statistical significance, and a post hoc Dunnett’s multiple-comparison test was used to determine pairwise significance compared with Nx control mice within each time point. NS, not significant (P > 0.05). *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.