Fig. 5.

Effect of the Rho kinase inhibitors Y-27632 and RKI-1447 on intracellular Ca²⁺ concentration ([Ca²⁺]_i) in rat cultured A7r5 aortic smooth muscle cells and rat preglomerular microvascular smooth muscle cells (PMVSMCs). Changes in [Ca²⁺]_i were estimated by fura-2 fluorescence [ratio of fluorescence at 340 nm to that at 380 nm (F340-to-F380 ratio)] before and after incubation with 10 µM Y-27632 (±Y) or 10 µM RKI-1447 (±RKI) in cultured rat aortic A7r5 smooth muscle cells or in PMVSMCs, respectively. Each bar represents the mean ± SE.