Fig. 2.

A: body temperature. Basal body temperature (core body temperature; before experiment) was the average of 3 days before the experiment, and there was no difference among groups. Average core body temperature on each day before 2,4-dinitrophenol (DNP) administration (pre) showed a small but statistically significant difference between the DNP and DNP + heat groups. This difference was not scientifically significant. Core body temperature after the first exposure (Heat #1) and second exposure (Heat #2) was increased by heat and DNP, respectively. Core body temperature at the 1-h rest period after the first heat exposure (Rest #1) and the 3-h rest period after the second heat exposure (Rest #2) showed no difference between the nontreated (NT) control group and each group. B: trend of the body weight (BW) change corrected by basal BW. Calculation of ΔBW/base BW of each mouse was performed with the following formula: (BW at each point – BW before the experiment)/BW before the experiment. The BW change at day 8 after heat and DNP exposure was not different among groups. C: water intake. Water intake during daytime was increased in the heat and DNP + heat groups compared with the NT control and DNP groups, respectively. Water intake during night was decreased in the DNP group compared with the NT control group. Total water intake was not changed between the NT control and heat groups or NT control and DNP + heat groups. n = 5 mice/group. *P < 0.05; **P < 0.01; ***P < 0.001.