Fig. 5.

A: Western blots of isolated mitochondria from kidney tissue. Mitochondrial aconitase-2, which represents mitochondrial function, was decreased in the 2,4-dinitrophenol (DNP) + heat group. Mitochondrial NADPH oxidase 4 (NOX4), which represents intramitochondrial oxidative stress, was increased in the DNP and heat groups. Cytochrome c oxidase complex IV (COX IV) was used as an internal control. B and C: quantification of intramitochondrial protein expression [aconitase-2 (B) and NOX4 (C)]. The intensity and area of bands were analyzed by ImageJ and standardized by COX IV. n = 4 mice. *P < 0.05; ***P < 0.001.