Acoustic responses in Bhlhb5-ChR2R mice. A: click-evoked auditory brainstem response (ABR) waveforms (I–V) from homozygous (Homo; green), heterozygous (Hetero; orange), and control (Ctrl; black) littermates. B: ABR thresholds for pure tone test from homozygous (n = 5), heterozygous (n = 6), and control (n = 5) mice. C–E: amplitudes, latencies, and widths at half-height (Whalf) of acoustically evoked ABR peak 1 as a function of click intensity. The number of ears from each group was the same as in B.