Fig. 3.

Effects of acetaldehyde on mitochondrial fusion-related proteins in SH-SY5Y cells. SH-SY5Y cells were exposed to various concentrations of acetaldehyde (0, 4, 20, 100, 500 μM) for 24 h. The protein levels of Mfn1 and OPA1 in the cell lysates were determined by Western blot analyses. The intensities of the bands were quantified by densitometric analyses and normalized by the amount of actin. Data represent the mean ± SEM of 3 independent experiments.