Fig. 4.

Acetaldehyde promotes Drp1 phosphorylation at Ser616 and the translocation of Drp1 to mitochondria. SH-SY5Y cells were exposed to acetaldehyde (500 μM) for the indicated period of time (A) or at various concentrations (0, 4, 20, 100, 500 μM) for 2 h (B). Whole cell lysates were prepared and the protein levels of p-Drp1 (Ser616) and Drp1 were determined by Western blot analyses. (C) SH-SY5Y cells were exposed to acetaldehyde at various concentrations (0, 4, 20, 100, 500 μM) for 24 h. The mitochondrial and cytoplasmic fractions were prepared and the protein levels of p-Drp1 (Ser616) and Drp1 were determined by Western blot analyses. Tubulin and Tim23 were used as internal control of cytoplasm and mitochondria, respectively. The intensities of the bands were quantified by densitometric analyses and normalized by the amount of Drp1 or actin as indicated in the graphs. Data represent the mean ± SEM of 3 independent experiments. *p < 0.05 versus control.