Fig. 6.

ROS-mediated activation of JNK and p38 MAPK is important for Drp1 phosphorylation induced by acetaldehyde. SH-SY5Y cells preincubated with NAC (1 mM) for 1 h were treated with acetaldehyde (500 μM) for 2 h. The levels of p-JNK (Thr183/Tyr185) (A) and p-p38MAPK (Thr180/Tyr182) (B) were determined by Western blot analyses. (C) SH-SY5Y cells were preincubated with sp600125 (20 μM) or sb203580 (5 μM) (D) for 0.5 h, then treated with acetaldehyde (500 μM) for 2 h. The levels of p-Drp1 (Ser616) were determined by Western blot analyses. The intensities of the bands were quantified by densitometric analyses and normalized by the amount of JNK, p38MAPK or Drp1. Data represent the mean ± SEM of 3 independent experiments. *p < 0.05 versus control; ^#p < 0.05 versus acetaldehyde-treated group.