Fig. 4.

(a) Duration of the expression level of Fluc in the liver (circle) and Gluc in plasma (inverted triangle) 6, 24, and 48 h after hydrodynamics-based intravenous injection of naked pDNA in mice. Each point represents the mean ± S.E. at least 10 experiments. Tukey multiple comparison tests were performed (**, P < 0.01; ***, P < 0.001 vs. 6 h group of Fluc; #, P < 0.05 vs. 6 h group of Gluc). (b) Duration of log-Fluc/log-Gluc ratios. Each point represents the mean ± S.E. at least 10 experiments. Tukey multiple comparison test was performed (***, P < 0.001 vs. 6 and 48 h groups). (c) Correlation between Fluc activity in the liver and Gluc activity in plasma 6 (Circles), 24 (Squares) and 48 h (Triangles) after hydrodynamics-based intravenous injection of naked pDNA in mice. Symbols and lines express luciferase expression levels in individual mice and regression lines, respectively.