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. 2019 Nov 26;18:1533033819887962. doi: 10.1177/1533033819887962

Figure 3.

Figure 3.

BRAF-activated noncoding RNA directly targets miR-195-5p. (A) The predicated miR-195-5p binding site of BANCR (CCAT1-Wt) and the designed BANCR-Mut are indicated. (B) Relative expression of miR-195-5p in PC tissues and adjacent healthy tissues was analyzed by qRT-PCR (n = 45, **P < .01). (C) Relative expression of miR-195-5p in PC cell lines and HPNE cells was measured by qRT-PCR. The level of relative miR-195-5p expression was decreased in 4 PC cell lines, PANC-1, SW1990, HS766T, and CFPAC-1, compared to HPNE. *P < .05 and **P < .01 versus HPNE group. (D) MiR-195-5p was negatively correlated with BANCR expression. (E) Luciferase assay was used to verify the binding between miR-195-5p and BANCR. **P < .01 versus miR-NC group. (F-G) Endogenous BANCR pulldown by Ago2 upon overexpression of miR-195-5p was determined using RIP assays. **P < .01 versus miR-NC group. (H-I) PANC-1 and SW1990 cells were transfected with si-BANCR1 and si-BANCR2; the expression of miR-195-5p was examined using qRT-PCR. **P < .01 and ***P < .001 versus si-NC group. BANCR indicates BRAF-activated noncoding RNA; PC, pancreatic cancer; qRT-PCR, quantitative real-time polymerase chain reaction.