TABLE 2.
Sequences of the primers used in this study
| Target | Primer orientation | Primer name | Sequence (5′–3′) or reference |
|---|---|---|---|
| ERV-DC14/F. s. silvestris provirus | Forward | Fe-603S | AGTTAAGGGACTGTGGACTT |
| Reverse | Fe-587R | GCTGGGCATTGTTCTCCTTT | |
| pFUΔss DC14 env | Forward | Fe-610S | GGATCCGGATCCCACCATGAAACCCCCAGCGGGAAT |
| Reverse | Fe-184R | GAATTCGAATTCTATTCGATTGTATCTGGCCTTT | |
| ERV-DC14/F. s. silvestris/K148E | Forward | Fe-619S | GCATCGCCAGTAGTCTCGCAGCCCCATGCCG |
| Reverse | Fe-597R | CGGCATGGGGCTGCGAGACTACTGGCGATGC | |
| ERV-DC14/F. s. silvestris/S273P | Forward | Fe-620S | GGTGTCTGAGTTGGGGCCCTTGGTGGC |
| Reverse | Fe-598R | GCCACCAAGGGCCCCAACTCAGACACC | |
| pFUΔss Ampho-MLV env mutant | Forward | Fe-636S | GGTAAATGGGGGTGTAAAACCACCGGACAGG |
| Reverse | Fe-614R | CCTGTCCGGTGGTTTTACACCCCCATTTACC | |
| pFUΔss Friend MLV env mutant | Forward | Fe-637S | CCTCTTGGGGCTGCAAGACAACCGGTAGA |
| Reverse | Fe-615R | TCTACCGGTTGTCTTGCAGCCCCAAGAGG | |
| pFUΔss FeLV-A env mutant | Forward | Fe-638S | GCTGCATGGGGTTGCAAAACTACGGGAGAAG |
| Reverse | Fe-616R | CTTCTCCCGTAGTTTTGCAACCCCATGCAGC | |
| pFUΔss FeLV-B env mutant | Forward | Fe-639S | CTGTATGGGGTTGCAAGACCACCGGGGAA |
| Reverse | Fe-617R | TTCCCCGGTGGTCTTGCAACCCCATACAG | |
| ERV-DC14/pol-3′fla | Forward | Fe-501S | AAGGAATTGCCAAAGGAGTTCTAA |
| Reverse | Fe-587R | GCTGGGCATTGTTCTCCTTT | |
| pFUΔss DC7 env | Forward | Fe-650S | GGATCCGGATCCCACCATGAAACCCCCAACAGGAAT |
| Reverse | Fe-626R | GCGCGAATTCGAATTCTCATTCGATTGTATCTGGCC | |
| ERV-DC14 5′ LTR | Forward | Fe-603S | AGTTAAGGGACTGTGGACTT |
| Reverse | Fe-140R | ACAAAACATAGAACACAATACC | |
| ERV-DC7/F. s. silvestris provirus | Forward | Fe-66S | CCGAAAAMTTCCTGACTGTTTAAGA |
| Reverse | Fe-53R | AGAGGAAATAAACCGGGTAGTGTGT | |
| ERV-DC16/F. s. silvestris provirus | Forward | Fe-219S | GCCACGGTCATGAAAATAAAAA |
| Reverse | Fe-44R | TGCAGACAGAACATACTGTGACAAA |