FIG 2.

Visualization of EBV pentamer-binding cells in brain sections from HLA-B*0801⁺ MS donors. Serial brain sections from two HLA-B*0801⁺ MS donors were stained with MAbs to CD8 and CD20 and with EBV-peptide coupled pentamers. (A and B, donor MS234) Perivascular cuff containing CD8 T cells (A) and some cells binding the B*0801/EBNA3A pentamer (B) in an active WM lesion. (C to G, donor MS121) Large perivascular cuff containing scattered CD8 T cells (C), tightly packed CD20 B cells (D), and several cells binding the B*0801/BZLF1 pentamer (E; panel F shows the area within the frame in panel E at high-power magnification) at the periphery of an active WM lesion; in the same cuff, no staining is observed after incubation with HLA-mismatched A*0201/LMP2 pentamer (G). Nuclei were stained with hematoxylin. Bars, 100 μm (D and G) and 50 μm (A to C, E, and F).