TABLE 3.
Percentages of CD8 T cells recognizing EBV, CMV, influenza A virus, and MBP protein-derived peptides in the total CD8 T-cell population infiltrating the MS brain
| Donor ID and HLA class I allele | Brain tissue block analyzed | % of pentamer-binding cells in the total CD8 population |
|||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EBV |
CMV |
Influenza A virus |
MBP | ||||||||||||||
| EBNA3A | EBNA3C | LMP1 | LMP2 | BZLF1 | BRLF1 | BMRF1 | BMLF1 | BALF4 | Sum of % of EBV pentamer- binding cellsa |
EBNA3A + BZLF1b |
pp65 | IE1 | MP | NP | |||
| MS79 | |||||||||||||||||
| A*0201 | A3B5 | 0.9 | 1.0 | 1.7 | 3.6 | 0.3 | 0 | 0 | |||||||||
| A1D5 | 1.3 | 0.8 | 1.1 | 0.8 | 1.7 | 5.7 | |||||||||||
| A1B4 | 2.7 | 1.8 | 1.7 | 1.8 | 0.7 | 8.7 | |||||||||||
| MS286 | |||||||||||||||||
| A*0201 | A1A3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |||||||||
| A1C1 | 0 | 0 | 0 | 0 | 0 | ||||||||||||
| MS330 | |||||||||||||||||
| B*0702 | A6A1 | 1.9 | 1.0 | 0.6 | 3.5 | 0 | 0 | ||||||||||
| MS356 | |||||||||||||||||
| B*0702 | A3B4 | 1.0 | 0.8 | 0 | 1.8 | 0 | 0 | ||||||||||
| MS289 | |||||||||||||||||
| A*0201 | A1A2 | 1.7 | 1.7 | 0 | 1.7 | 4.1 | 0 | 19.2 | 0 | 0 | 0 | ||||||
| B*0702 | 2.0 | 3.5 | 4.5 | 0 | 0 | ||||||||||||
| A*0201 | P2B7 | NAc | 1.8 | 0 | 1.3 | 3.8 | 1.6 | 12.5 | 0 | 0 | 0 | ||||||
| B*0702 | NA | 0.9 | 3.1 | ||||||||||||||
| MS342 | |||||||||||||||||
| A*0201 | P1C1 | 0.3 | 1 | 0.8 | 0 | 0.4 | 0.5 | 3.6 | 0 | 0 | 0 | ||||||
| B*0702 | 0.2 | 0.2 | 0.2 | 0 | |||||||||||||
| MS92 | |||||||||||||||||
| B*0801 | A5B2 | 0.5 | 2.2 | 2.7 | 0.2 | ||||||||||||
| MS121 | |||||||||||||||||
| B*0801 | P3D4 | 0.2 | 2.7 | 2.9 | 0 | ||||||||||||
| MS154 | |||||||||||||||||
| B*0801 | A2E3 | 0 | 3.8 | 3.8 | 3.8 | 0 | |||||||||||
| MS180 | |||||||||||||||||
| B*0801 | P1B4 | 2.0 | 3.1 | 5.1 | 0 | ||||||||||||
| A1D7 | 0.4 | 1 | 1.4 | 0 | |||||||||||||
| MS234 | |||||||||||||||||
| B*0801 | P4C3 | 1.6 | 3.5 | 5.1 | 9.0 | 0.2 | |||||||||||
| MS352 | |||||||||||||||||
| B*0801 | P1B5 | 1.8 | 1.3 | 3.1 | 7.7 | 0.2 | |||||||||||
| P5C3 | 0.1 | 1.8 | 1.9 | 0 | |||||||||||||
a
The figures in this column were obtained by summing the percentages of cells binding different EBV protein-derived peptides in serial sections from the same brain tissue block.
b
The figures in this column represent the percentages of pentamer-binding cells in brain sections incubated simultaneously with EBNA3A/B*0801 and BZLF1/B*0801 pentamers.
c
NA, not assessable.