FIG 2.
Identification of CARD11, a host factor with brain-specific upregulation in NDV-infected chickens. (A) The numbers of DEGs with different FCs (P < 0.05) in virus-infected brains are shown in the columns. Four-week-old SPF chickens were infected with virulent F48E9 and avirulent LaSota (105 PFU/100 μl) via the intraocular-nasal route. Brain samples were collected at 5 dpi. Total RNA was extracted from the brains for gene microarray analysis. The relative mRNA expression of CARD11 in NDV-infected chicken brains (B) and other tissues (C) normalized to that of 28S rRNA was determined by RT-qPCR. (D) The relative mRNA expression of IRF1, TLR15, TMEM173, and TNFRSF8 in NDV-infected chicken tissues was determined by RT-qPCR and normalized to that of 28S rRNA. Representative data shown as the means ± SDs (n = 3) were analyzed by two-tailed Student's t tests. **, P < 0.01.