FIG 5.

The infection efficiency of rAdVs in chPNCs. (A) Detection of rAdV-CARD11 (MOI = 100) infected HEK293A cells and chPNCs. At 36 hpi in HEK293A cells and 48 hpi in chPNCs, the cell lysates were analyzed by Western blotting with an anti-Flag mouse MAb. β-Tubulin was used as a protein loading control. (B and C) Infection efficiency of rAdV-CARD11 in chPNCs. The chPNCs were infected with rAdV-CARD11 at different MOIs (1, 10, 50, 100, and 200) for 2 h at 37°C. The rAdV-CARD11-infected chPNCs at 48 hpi were collected by 0.25% trypsin digestion and incubated with an anti-Flag mouse MAb and goat anti-mouse IgG/FITC at 4°C with minimal exposure to light. (B) After the cells were washed, they were assessed by flow cytometry. (C) The cells were observed under a microscope. The black arrow indicates cell bodies with no axons. Bars, 50 μm.