FIG 1.

HSV-1 SC16 reactivation and viral DNA from TG harvested during latency. BALB/c mice were inoculated in the left upper lip with wild-type HSV-1 SC16. At 28 DPI (i.e., a time known to correspond with latent phase of TG infection in the OO model), mice were sacrificed. (A) TG from mice (n = 12) were harvested and incubated individually in culture medium (CM) for 14 days. Aliquots of medium were removed every 2 days and compensated with fresh CM. Infectious particle release (reactivation) from aliquots was measured by plaque assay. Bars represent the percentages of TG cultures from individual mice in which plaques developed at any given time. **, P < 0.01 (Kruskal-Wallis test). (B) DNA was extracted and qPCR performed targeting the gG coding sequence or mouse GAPDH gene, according to the method of Cavallero et al. (22). Results were determined as the average numbers of gG genome copies relative to GAPDH DNA, and bars represent the 95% confidence intervals. ns, not significant.