FIG 3.

The T_EM subset is characterized by expression of markers of cell cycle entry and of T cell activation. (a to c) Percentages of CD4⁺ T cells in the T_CM/T_TM subsets (blue circles) and T_EM subsets (red circles) in resting phase (G₀) (a), growth phase (G₁) (b), and DNA synthesis phase (S) and mitosis phase (M) (c) of the cell cycle from virally suppressed HIV-infected individuals (bars indicate means with SD from a Wilcoxon matched-pair signed-rank test [n = 17]). (d) Percentages of cells in the cycle are represented with the equation [(G₁ + G₂ + M + S)/(G₀ + G₁ + G₂ + M + S)] × 100, which shows significantly more cycling cells in the T_EM subset than in other subsets (bars indicate means with SD from a Wilcoxon matched-pair signed-rank test [n = 17]). (e to i) Percentages of cells in the T_CM (blue circles), T_TM (orange circles), and T_EM (red circles) subsets positive for HLA-DR (n = 45) (e) or coexpressing CD38/HLA-DR (n = 45) (f), PD-1 (n = 45) (g), Ki67 (n = 57) (h), or CD127 (n = 40) (i). P values are indicated from a Mann-Whitney test. Measurements were based upon the availability of cells from each cohort.