FIG 2.
Chemical structures of different CD4mc and their capacity to expose the CoRBS. (A) (S)-MCG-II-153 shares some characteristics with CD4mc NBD-556 (17). Based on analogy to (+)-BNM-III-170, a fluorine group was added to (S)-MCG-II-153 to make (S)-MCG-III-027-D05. (B) The CBE was used to evaluate 17b binding to HIV-1JR-FL EnvΔCT expressed at the surface of HOS cells in the presence of the different compounds (50 μM), sCD4 (10 μg/ml), or the compounds’ vehicle (DMSO). Data shown are the mean RLU values ± SD from at least three independent experiments performed in quadruplicates, with the signal obtained from wells transfected with an empty pcDNA3.1 plasmid (no Env) subtracted, normalized to Env levels as determined by 2G12 binding. Statistical significance was evaluated using an unpaired t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not significant).