FIG 5.
MCG analogs contact highly conserved residues D368 and E370. A CBE was used to determine CoRBS Ab 17b binding to WT HIV-1JR-FL EnvΔCT or mutants with different alterations, S375W, D368A, D368R, E370R, or D368R-E370R, expressed at the surface of HOS cells in the presence of CD4mc (50 μM) or the compounds’ vehicle (DMSO). Shown are the mean RLU values ± SD from at least three independent experiments performed in quadruplicates, with the signal obtained from wells transfected with an empty pcDNA3.1 plasmid (no Env) subtracted, normalized to Env levels as determined by bNAb 2G12 binding.