FIG 8.

(S)-MCG-IV-210 blocks uninfected bystander ADCC killing. Uninfected primary CD4⁺ T cells were stained with the cellular dye eFluor-450 and cocultured with unstained NL4-3.ADA.GFP WT virus-infected autologous cells for 72 h. (A) The ability of HIV⁺ plasma to recognize uninfected bystander cells in the presence of 50 μM (+)-BNM-III-170 or (S)-MCG-IV-210 was evaluated by FACS analysis. (B) These uninfected eFluor-450⁺ cells were also used as target cells for ADCC with autologous PBMCs and 5 HIV⁺ plasma samples in the presence of 50 μM (+)-BNM-III-170 or (S)-MCG-IV-210. Statistical significance was evaluated using a paired t test (*, P < 0.05; ****, P < 0.0001; ns, not significant).