FIG 4.

Suppression of DDX21 gene expression does not affect vRC formation or viral DNA synthesis. (A) MRC5 cells transduced with lentivirus respectively expressing shC, shDDX21-1, or shDDX21-3 were infected with HCMV at an MOI of 0.1. Cells were fixed at 72 hpi and stained with antibody against UL44, which is a protein known for localization of the vRC (viral replication compartment). (B) Knockdown of DDX21 had no effect on viral DNA synthesis. MRC5 cells respectively expressing shC, shDDX21-1, or shDDX21-3 were infected with HCMV at an MOI of 1. Intracellular DNA was extracted at the indicated times after infection. Viral DNA levels were quantified by qPCR, and the normalized gene is actin. The result for the DNA sample from shC-expressing cells collected at 2 h was set equal to 1.